Abstract:The development and characteristics of Lingnan flower arrangement are heavily influenced by the Lingnan School of paintings, which is closely related to the tradition of Chinese literati paintings. This paper compares flowers in vase in Lingnan literati paintings in the late Qing Dynasty and the Republic of China era with those in Jiangnan literati paintings, which is the most representative of literati art tradition in the same period. And then, the paper analyzes the similarities and differences of flowers in vase in the paintings between the two regions in terms of floral materials, flower types, combinations, creative intentions and styles, and explores the regional characteristics of flowers in vase in the paintings in Lingnan and Jiangnan. The flowers in vases painted by literatus of Lingnan and Jiangnan both reflect traditional Chinese wisdom and literati aesthetics, and both are influenced by civic culture. The difference lies in the fact that literatus in Lingnan focus on showing people’s daily life and the paintings are rich in life interest, while literatus in Jiangnan take literati interest as the basis and emphasize the beauty of the branches and the mood of the work.