Abstract:Identifying cultural-ecological region boundaries scientifically and clarifying the relationship between region, culture and ecology is the primary issue of holistic protection of cultural ecology. Combined with the actual situation of Li-Miao culture in Hainan Island, the analytic hierarchy process is used to select indicators from the three levels of culture, ecology and economy, build the Li-Miao cultural ecological index system,calculate the connection degree of urban cultural ecological development by using the gravity model, define the scope of Li-Miao cultural ecological area in Hainan Island as nine cities in the central and southern region of Hainan Island with Wuzhishan, Sanya and the East as the core, then it is divided into three sub regions: central core area, southern optimization development area and Western integration and upgrading area. And integrated cultural and ecological areas within the development of supporting capacity and regional factor analysis, it is found that there is a clear imbalance between resource distribution and supporting capacity, development linkage strength is not enough in Hainan Island Li-Miao cultural and ecological areas.